20040-036 Q Ceramic HyperD 20 Chromatography Resin 100mL |
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3181 |
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Iyem No:20040-036 |
Product Nmae:Q Ceramic HyperD 20 Chromatography Resin 100mL |
Package Unit:100mL |
Mark:Pall Corporation |
Price:Contact us |
RelateDate :Web site |
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Pall
Chromatography Products-Resins,Kits and Prepacked Columns
層析產品系列
依純化方式的不同,Pall的層析類產品可分為五大類:
1.依分子體積分餾與純化(Size Exclusion)-Ultrogel AcA & Trisacryl GF
用途:依分子體積分餾與純化;分子量的判定;去鹽。
特色:使用簡單,不會使蛋白質變性而影響其活性。
規格:
服服 |
Particle Size |
Capacity |
Exclusion Limit |
Fracrionation Range |
Ultrogel AcA 22 |
60-140μm |
n.a. |
3,000 KD |
100-1,200 KD |
Ultrogel AcA 34 |
750 KD |
20-350 KD |
Ultrogel AcA 44 |
200 KD |
10-130 KD |
Ultrogel AcA 54 |
90 KD |
5-70 KD |
Ultrogel AcA 202 |
Desalting capacity 45% gel volume |
22 KD |
1-15 KD |
Trisacryl GF05 |
註1 |
Desalting capacity 33% gel volume |
3 KD |
200-2,500 D |
Trisacryl GF2000 |
n.a. |
20,000 KD |
10-15,000 KD |
註1:GF05與GF2000均分為M grade 與LS grade,設計為實驗室使用的M grade濾珠大小差距較小(40-80μm);而設計供產程應用的LS grade(80-160μm)適合於壓力下以高流速進行分餾(最大可承受3bar的壓力)。 |
2.以所帶電荷的親和力進行分離(Ion Exchange)-Q, S, DEAE & CM Ceramic HyperD Resins 陰離子交換 (陶瓷珠本身帶正電);Q (強) & DEAE (弱),陽離子交換 (陶瓷珠本身帶負電);S (強) & CM (弱)。
用途:蛋白質、多月太 (polypeptide)或質體的濃縮與純化。
特色:本類產品均由Pall專利陶瓷珠製成,不僅較傳統resin耐壓軸流速↑),且binding capacity亦高。
規格:
Product |
Particle Size |
Capacity |
Pressure Stability |
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Q Ceramic HyperD 20 |
20μm(avg) |
BSA:>85mg/ml |
200bar |
S Ceramic HyperD 20 |
Lysozyme:>85mg/ml |
Q Ceramic HyperD F |
50μm(avg) |
BSA:>85mg/ml |
70bar |
S Ceramic HyperD F |
Lysozyme:>85mg/ml |
DEAE Ceramic HyperD F |
BSA:>85mg/ml |
CM Ceramic HyperD F |
IgG:>60mg/ml |
3.依物質對 resin 上特定配體 (ligand) 的親和力進行純化 (Affinity)
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Product |
Ligand |
Capacity |
Application |
RESIN |
Blue Trisacryl M |
Cibacron Blue F3GA chromophore |
HSA:10-15mg/ml BSA:5-7mg/ml |
Albumin depletion, purification of enzymes and proteins such as kinasws, interferons and some coagulation factors |
Protein A Ceramic HyperD F |
Recomophore |
Human IgG:>30mg/ml |
IgG purification/depletion |
Heparin HyperD M |
Porcine Heparin |
Human ATIII:>25mg/ml |
Purification of coagulation factors, lipoproteins, growth hormones, growth factors, nucleic acid binding enzymes |
IMAC HyperCel" |
Metal Ions, such as CU++,Zn++, Co++, Ni++, GA+++, or FE+++ |
Metal ion capacity:40-70μmol Cu++/ml,Ionic capacity:90-140μeq/ml |
Purification of HIS-tagged proteins, antibodies and prefrationation of comples protein mixtures |
Lysine HyperD |
Immobilized L-lysine |
n.a. |
Purification of glycoproteins and other biological molecules that bind to Lysine |
4.利用兩種以上的特性進行分離與純化(Mixed Mode)
SDR HyperD |
MEP HyperCel |
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Product |
Mwthods |
Capacity |
Application |
MEP HyperCel |
Hydrophobic Ineraction Electrostatic Repulsion |
Human IgG:>20mg/ml |
Purification/depletion of polyclonal and monoclonal antibodies from most species |
SDR HyperD |
Hydrophilicity, Hydrophilicity & Size Exclusion (exclusion limit:10kDa) |
Triton X-100:60-80mg/ml |
Solvent-Detergent Removal while recovering NATIVE protein |
HEA HyperCel |
Hydrophobic Interaction Electrostatic Repulsion |
BSA:40-60mg/ml (Recovery>90%) |
Unique industry-scalable sorbents designed foe protein capture and impurity removal in a abiopharmaceutical environment. Provides different selectivites not accessible with traditional ion exchange or HIC. |
PPA HyperCel |
5.Hu Ultrogel
用途:蛋白質、胜月太與核酸的分離與純化,尤其是鹼性蛋白質(cytochrome c, lysozyme)與磷酸化蛋白質。
特色:為Hydroxyapatile的微晶體鑲於agarose bead中。
Product |
Particle Size |
Capacity |
HA Ultrogel |
60-180μm |
Cytochrome C:>7mg/ml BSA:<7mg/ml |
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